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KMID : 1024520100190091077
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Journal of the Environmental Sciences
2010 Volume.19 No. 9 p.1077 ~ p.1082
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Biosynthesis and Control of Keratinase in Recalcitrant Feather-Degrading Bacillus megaterium F7-1
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Jeong Jin-Ha
Lee Na-Ri
Jeon Young-Dong
Kim Jeong-Do
Park Ki-Hyun
Park Geun-Tae
Son Hong-Joo
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Abstract
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This study was performed to investigate the nutritional conditions controlling keratinase activity in Bacillus megaterium F7-1. B. megaterium F7-1 produced keratinase using chicken feather as a sole source of carbon, nitrogen and sulfur. Addition of the feather medium with glucose enhanced keratinase production (68.9 U/ml), compared to control without glucose (63.2 U/ml). The synthesis of keratinase was repressed by addition of NH4Cl in B. megaterium F7-1. The highest keratinase production (70.9 U/ml) was obtained with the feather medium containing glucose and MgSO4·7H2O. Keratinase was produced in the absence of feather (4.9 U/ml), indicating its constitutive synthesis. Feather degradation resulted in free SH group formation. B. megaterium F7-1 effectively degraded chicken feather meal (86%), whereas duck feather, human nail, human hair and sheep wool displayed relatively low degradation rates (8-34%).
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KEYWORD
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Bacillus megaterium, Feather, Keratinase, Biosynthesis control
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